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Guido-Garcia, F.; Sakamoto, Fuminori; Kozai, Naofumi; Grambow, B.
no journal, ,
Cs was released from FNPP in 2011 and is one of the main concerns due to its half-life and prevalence in forests. Here, Cs can be taken and accumulated by different organisms, e.g. mushrooms. This can result in mobilization through the environment. Elucidating the mechanisms by which cesium is taken from soil by mushrooms is key to understand its environmental behavior. In soil, Cs can be tightly fixed to clay minerals thus its bioavailability is poor. Organic acids and siderophores produced by mushrooms may remove Cs from mineral surfaces making it bioavailable. To assess this, different strains of fungi were chosen from a ~1000 collection that has been previously tested for Cs accumulation capacity. Organic acids were analyzed by GC-MS and HPLC-ICP-MS from different strains with different accumulating capacities. These were then tested in cesium desorption experiments using key minerals, and compared to a commercial siderophore.
Sakamoto, Fuminori; Guido-Garcia, F.; Kozai, Naofumi; Tanaka, Kazuya; Grambow, B.
no journal, ,
The radiocesium becomes the stress for creatures, but it is known that some plants and fungi collect radiocesium. We continue a study using a mushroom for the purpose of decontamination of the radiocesium by microbes. We tried to confirm species specificity, expressed proteins and so on to elucidate the mechanism of accumulation of radiocesium by mushroom. As a result, we identified 10 species that accumulate Cs as a high rate and another 10 species that accumulate Cs as a low rate. We checked the specificity of species, but any tendencies, regularity and specificity were not discovered. We only confirmed the tendency that high accumulated species need a long culture period and low accumulated species need not a long culture period. As a result of two-dimensional electrophoretic analysis, we specified 4 kinds of proteins which express under Cs existence and 5 kinds of proteins which disappeared under Cs absence.